講座預告

- 校內新聞熱線:9291819
報告題目:細胞壁多糖合成的酶學探究
報告人:曾為(墨爾本大學)
時間:2016年9月22日(周四)上午9:00
地點:智能實驗樓學術報告廳
報告人簡介:
曾為,湖南湘潭人。2001年本科畢業于北京師范大學生命科學學院。2009年博士畢業于美國俄亥俄大學(Ohio University)細胞和分子生物學專業。現在澳大利亞墨爾本大學(University of Melbourne)植物細胞壁研究中心(ARC Centre of Excellence in Plant Cell Walls) Tony Bacic研究組工作。植物細胞壁多糖由糖基轉移酶(Glycosyltransferase)參與合成,但其合成分子機理尚不清楚。其研究內容主要是運用分子生物學和生物化學分析植物糖基轉移酶的功能,使用外源系統表達植物糖基轉移酶并設計酶學反應,在試管中模擬植物多糖的合成,并用高效液相色譜(HPLC),質譜(Mass Spectrometry),核磁共振(NMR)等技術手段對產物進行鑒定。已在木聚糖(Xylan) 合成研究中取得一定突破,探索了植物細胞壁合成機理,對進一步改造植物細胞壁,讓其更好服務人類提供了理論基礎。
Publications:
1. Zeng, W.; Chatterjee, M.; Faik, A. Udp-xylose-stimulated glucuronyltransferase activity in wheat microsomal membranes: Characterization and role in glucurono(arabino)xylan biosynthesis. Plant physiology 2008, 147, 78-91.
2. Zeng, W.; Jiang, N.; Nadella, R.; Killen, T.L.; Nadella, V.; Faik, A. A glucurono(arabino)xylan synthase complex from wheat contains members of the GT43, GT47, and GT75 families and functions cooperatively. Plant physiology 2010, 154, 78-97.
3. Li, L.; Huang, J.; Qin, L.; Huang, Y.; Zeng, W.; Rao, Y.; Li, J.; Li, X.; Xu, W. Two cotton fiber-associated glycosyltransferases, GhGT43a1 and GhGT43c1, function in hemicellulose glucuronoxylan biosynthesis during plant development. Physiologia plantarum 2014, 152, 367-379.
4. Zhao, X.; Ouyang, K.; Gan, S.; Zeng, W.; Song, L.; Zhao, S.; Li, J.; Doblin, M.S.; Bacic, A.; Chen, X.Y., et al. Biochemical and molecular changes associated with heteroxylan biosynthesis in neolamarckia cadamba (rubiaceae) during xylogenesis. Frontiers in plant science 2014, 5, 602.
5. Ford, K.L.; Zeng, W.; Heazlewood, J.L.; Bacic, A. Characterization of protein n-glycosylation by tandem mass spectrometry using complementary fragmentation techniques. Frontiers in plant science 2015, 6, 674.
6. Song, L.; Zeng, W.; Wu, A.; Picard, K.; Lampugnani, E.R.; Cheetamun, R.; Beahan, C.; Cassin, A.; Lonsdale, A.; Doblin, M.S., et al. Asparagus spears as a model to study heteroxylan biosynthesis during secondary wall development. PloS one 2015, 10, e0123878.
7. Kuang, B.; Zhao, X.; Zhou, C.; Zeng, W.; Ren, J.; Ebert, B.; Beahan, C.T.; Deng, X.; Zeng, Q.; Zhou, G., et al. The role of udp-glucuronic acid decarboxylase (UXS) in xylan biosynthesis in arabidopsis. Molecular plant 2016, 9, 1119-1131.
8. Zeng, W.; Lampugnani, E.R.; Picard, K.L.; Song, L.; Wu, A.M.; Farion, I.M.; Zhao, J.; Ford, K.; Doblin, M.S.; Bacic, A. Asparagus IRX9, IRX10, and IRX14A are components of an active xylan backbone synthase complex that forms in the Golgi apparatus. Plant physiology 2016, 171, 93-109.
9. Ford K.; Chin T.; Srivastava V.; Zeng W.; Doblin M.; Bulone V.; Bacic A. Comparative “Golgi” proteome study of Lolium multiflorum and Populus trichocarpa. Proteomes 2016, 4, 23.
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林業與生物技術學院
2016年9月21日